Unexpected low oxygen tension of intravaginal culture

Does embryo culture at low oxygen tension improve ART outcomes?

Even with the endogenous defense mechanisms present in oocytes and embryos, disturbances in embryonic culture processes may lead to an increased generation and buildup of reactive oxygen species (ROS) in culture media. This fact may be associated with various degrees of cellular damage (e.g. membrane disturbances, DNA fragmentation, etc.). Consequently, embryonic fragmentation, apoptosis, or ev...

IVF/ICSI outcomes after culture of human embryos at low oxygen tension: a meta-analysis

BACKGROUND Improved pregnancy, implantation, and birth rates have been reported after the use of reduced O2 concentration during embryo culture, mainly due to a reduction of the cumulative detrimental effects of reactive oxygen species. However, some studies have failed to report any positive effects. The objective of this meta-analysis was to evaluate the effect of a low-O2 environment on IVF/...

Oxygen consumption of equine articular chondrocytes: Influence of applied oxygen tension and glucose concentration during culture.

We investigated the oxygen (O(2)) uptake of equine articular chondrocytes to assess their reactions to anoxia/re-oxygenation. They were cultured under 5% or 21% gas phase O(2) and at glucose concentrations of 0, 1.0 or 4.5g/L in the culture medium (n=3). Afterwards, the O(2) consumption rate of the chondrocytes was monitored (oxymetry) before and after an anoxia period of 25min. The glucose con...

Low oxygen tension in the management of newborn infants.

Prolonged administration of oxygen in high concentrations to premature infants is now being abandoned in most countries, thanks to the studies on retrolental fibroplasia which have shown its harmful effects on the eyes. These studies have led to a revision of the indications for oxygen therapy and prompted renewed interest in its physiological bases in the neonatal period (Engle and Levine, 195...

Tissue metabolism under the influence of low oxygen tension.